Hello!

We conducted a bioequivalence study with 2 different formulations in 4-period design as follows:

First two periods (1 and 2) tested test (A) against reference ( B formulation in __fasting state__, last two periods (3 and 4) tested the same test (labelled as C) against the same reference (labelled as D) in __fed state__.

I wanted to check the food effect on the test formualtion and to perform ANOVA on A vs. C. I excluded Treatment B and Treatment D so in the analysis there were two treatments (A and C), 4 periods (in pairs foe each subjects 1 and 3 or 2 and 4 as in accordance with the respective sequence) and 2 sequences (ABCD and BADC).

ANOVA model used: Sequence+Treatment+Period+Subject(Sequence)

I didnt get any results in Average Bioequivalence (no LSMs, no anything ...). Why is this so?

On the other hand, if I exclude "Period" from the model, I get all results but is this model and results even correct? Or what would be the correct model in this case?

Edit: Additionally, if I put "Treatment+Sequence+Period(Treatment)+Subject(Sequence)" in the model, I also get some results (PEs are the same as in the model above without "Period", but CI are different). This is probably not a correct model but I was just trying to test different models in order to understand everything

Thank you

BEQool

**Edited by BEQool, 12 March 2024 - 01:24 PM.**